Molecular Cloning and Characterization of G Alpha Proteins from the Western Tarnished Plant Bug, Lygus hesperus

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Molecular Cloning and Characterization of G Alpha Proteins from the Western Tarnished Plant Bug, Lygus hesperus

The Gα subunits of heterotrimeric G proteins play critical roles in the activation of diverse signal transduction cascades. However, the role of these genes in chemosensation remains to be fully elucidated. To initiate a comprehensive survey of signal transduction genes, we used homology-based cloning methods and transcriptome data mining to identity Gα subunits in the western tarnished plant b...

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Characterization of microsatellite loci in the western tarnished plant bug, Lygus hesperus Knight (Hemiptera: Miridae)

A microsatellite-enriched partial genomic DNA library of Lygus hesperus was generated and screened by sequencing. Ten polymorphic microsatellite marker loci were characterized by genotyping 92 insect samples. The observed number of alleles ranged from three to seven with an average of 4.5 (SE ± 0.45), while the effective number of alleles ranged from 1.21 to 3.02 with an average of 2.14 (SE ± 0...

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Sequencing and De Novo Assembly of the Western Tarnished Plant Bug (Lygus hesperus) Transcriptome

BACKGROUND Mirid plant bugs (Hemiptera: Miridae) are economically important insect pests of many crops worldwide. The western tarnished plant bug Lygus hesperus Knight is a pest of cotton, alfalfa, fruit and vegetable crops, and potentially of several emerging biofuel and natural product feedstocks in the western US. However, little is known about the underlying molecular genetics, biochemistry...

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Oviposition Behaviors and Ontogenetic Embryonic Characteristics of the Western Tarnished Plant Bug, Lygus hesperus

The western tarnished plant bug, Lygus hesperus Knight (Hemiptera: Miridae) is a key pest of fruit, vegetable, and field crops in the western United States, but many aspects of L. hesperus ecology are poorly documented. A sound understanding of oviposition behavior and characterization of the phases of embryonic development would be useful in studies of interactions between L. hesperus and its ...

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Identification and characterization of a sex peptide receptor-like transcript from the western tarnished plant bug Lygus hesperus.

Lygus hesperus females exhibit a post-mating behavioural switch that triggers increased egg laying and decreased sexual interest. In Drosophila melanogaster, these changes are controlled by sex peptide (SP) and the sex peptide receptor (DmSPR). In Helicoverpa armigera, SPR (HaSPR) also regulates some post-mating behaviour; however, myoinhibiting peptides (MIPs) have been identified as the SPR a...

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ژورنال

عنوان ژورنال: Insects

سال: 2014

ISSN: 2075-4450

DOI: 10.3390/insects6010054